《植物生理学报》 2015, 51(4): 566-572

大白菜钙运输基因ECA和钙响应基因CAS在缺钙胁迫下的表达分析

程涣^1,2, 苏同兵², 于拴仓², 张凤兰², 余阳俊², 张德双², 赵岫云², 汪维红², 卢桂香², 龚义勤¹, 柳李旺^1,*

¹南京农业大学园艺学院, 南京210095; ²北京市农林科学院蔬菜研究中心, 北京100097

通信作者：柳李旺；E-mail: nauliulw@njau.edu.cn；Tel: 025-84395563

摘　要：

以干烧心抗性显著不同的两个大白菜自交系为材料, 研究了Ca²⁺运输基因ECA和Ca²⁺响应基因CAS在缺钙处理过程的表达变化。同源序列比对发现大白菜共有7个ECA基因和4个CAS基因; 缺钙处理后, 2个材料中的ECA基因都上调表达, 但在抗病材料中的上调更为显著; CAS基因在缺钙处理后的表达也有显著改变, 但各个CAS基因的表达模式表现较大差异。表明大白菜ECA和CAS基因的表达受钙胁迫影响, 并可能与大白菜苗期干烧心病的发生相关。

关键词：大白菜; 干烧心; Ca²⁺; 钙信号; 基因表达

收稿：2014-11-28   修定：2015-03-10

资助：科技部“973”项目(2012CB113906)、国家自然科学基金(31171970和31401875)、国家科技支撑计划(2012BAD-02B01)和大宗蔬菜产业技术体系(CARS-25-A-11)。

Expression Analysis of Ca²⁺ Transport and Response Genes, ECA and CAS, in Cabbage under Calcium Deficiency Condition

CHENG Huan^1,2, SU Tong-Bing², YU Shuan-Cang², ZHANG Feng-Lan², YU Yang-Jun², ZHANG De-Shuang², ZHAO Xiu-Yun², WANG Wei-Hong², LU Gui-Xiang², GONG Yi-Qin¹, LIU Li-Wang^1,*

¹College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; ²Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Corresponding author: LIU Li-Wang; E-mail: nauliulw@njau.edu.cn; Tel: 025-84395563

Abstract:

In this study, a total of 7 ECA and 4 CAS genes were identified in Chinese cabbage by protein sequence alignment. Phylogenetic analysis of ECA and CAS genes revealed that there were great differences between genes in the each gene family, which suggested that different genes might execute different functions. The expression of ECA and CAS genes was detected in tipburn-susceptive and resistant lines after Ca²⁺ deficiency treatment. The results showed that ECA genes were up-regulated in both lines, and expressed at a higher level in resistant plant than in sensitive plant. However, the expression pattern of different CAS genes was not consistent, which might be caused by functional division of different CAS genes. Our study suggested the expression of ECA and CAS was affected by calcium deficiency, and associated with tipburn occurrence in seedling of Chinese cabbage, which provided helpful information for further study the molecular mechanism of tipburn resistance in Chinese cabbage.

Key words: Chinese cabbage; tipburn; calcium; calcium signal; gene expression